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KMID : 0358219930200010053
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Korean Journal of Fertility and Sterility
1993 Volume.20 No. 1 p.53 ~ p.56
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A Study on Grwoth of Human Testicular Tissue in 3-Dimensional Collagen Gel Tissue Culture
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이충현
이상철/이선주/손준웅/장성구/김진일/채수용
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Abstract
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A recently developed collagen gel culture technique has been applied to study on growth in tissue of human testicular tissue. Minimum Eagle's medium supplemented with amino acid, 10% Fetal Bovine Serum and 0.1mM non-essential amino acid are
emploid.
Tissue fragments on collagan gel are fixed at time intervals for the histologic findings of testis. The mature spermatids are maintained for 2 weeks and can be observed until four weeks. But the rate of glucose consumption is increased contrary
to
histologic findings.
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KEYWORD
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